Bio-chemical Route Design

The integration of chemical and biocatalytic processing steps unlocks new potential in manufacturing capabilities by leveraging the strengths of both approaches. Enzymes, as exceptionally powerful catalysts, promote chemical transformations with efficiencies and selectivities that are otherwise challenging to achieve. Biocatalysis not only provides environmentally friendly and cost-effective alternatives to traditional chemical synthesis but also enables reactions that are chemically difficult, particularly when high stereoselectivity is required.

Below you can read more about our services.

Chemo-enzymatic Route Design

The pathway to successfully advancing your candidate. We combine innovative high-throughput screening strategies to quickly assess pathway feasibility and optimise factors such as enzyme selectivity, catalytic activity and product yield. We can also onboard your host to optimise production of your target molecule or to minimise the potential for bottlenecks or unwanted side reactions.

Our services include:

  • Retrosynthetic analyses, enabling us to break down the target molecule into smaller, simpler intermediates more likely to be found in metabolic pathways in nature or from readily available starting materials.
  • Development of new chemo-enzymatic and/or metabolic routes to substitute or compliment inefficient chemical synthesis strategies, preparing valuable, complex and challenging intermediates without costly functional group protection/deprotection approaches.
  • Competitive route risk assessment to establish more efficient, lower cost, practical and scalable synthetic routes to your candidate molecule.
  • Bioinformatic-based gene and biochemical pathway design provides efficient routes to (otherwise difficult to access) useful chiral synthons and advanced intermediates.
  • Rapid feasibility to determine biocatalytic potential to execute the desired substrate to product conversion.
Artistic representation of biochemical route design

Enzyme Discovery and Identification

Ingenza’s diverse array of biomanufacturing hosts support biocatalytic preparation of a variety of small molecules, especially where regio- and enantioselective control is required.

  • Bioinformatic, metagenomic and phylogenetic enzyme identification, isolation and screening in vivo or in vitro.
  • Identification of sequence-diverse structural homologues from public or proprietary databases.
  • Recombinant production and assessment of novel enzymes and/or metabolic pathways establishing novel customer foreground IP.
Petri dishes