Success story

Exquisitely selective enzymatic glycosylation

Customer challenge

Small molecule pharmaceutical requiring regio-selective glycosylation at only one of four possible sites in the molecule. Required multiple chemical protection/deprotection reactions to overcome poor yield of the desired product and a high level of by-products.

Ingenza’s inGenius® solution

Rapid identification of a suitable biocatalyst to provide regio-selective glycosylation at only the desired site in the molecule. Recombinant biocatalyst production plus optimisation of reaction conditions using Design-of-Experiment principles.

100%

100% regio-selectivity of the biocatalyst

>50x

Greater than 50x yield gain

80%

80% reduction in chemical processing steps

Delivered

Biotransformation process yielding over 20g/L titre of desired product with desired regioselective glycosylation. Avoidance of regio-isomer by-products. Technology transfer of process.